Method for purifying edible mushroom strain through broken inclined plane method

The invention provides a method for purifying an edible mushroom strain through a broken inclined plane method. Comprising the following steps: 1, tissue separation; step 2, mycelium culture; and step 3, carrying out tube transfer purification. According to the method, to-be-separated edible mushroo...

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Hauptverfasser: ZHANG GUOLI, GAO NENG, ZHU ZONGCAI, SUN YIFEI, CUI BAOKAI, WU DONGMEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for purifying an edible mushroom strain through a broken inclined plane method. Comprising the following steps: 1, tissue separation; step 2, mycelium culture; and step 3, carrying out tube transfer purification. According to the method, to-be-separated edible mushroom tissue blocks are firstly cultured on the PDA plate culture medium until hyphae germinate, then tip hyphae are picked and inoculated to the middle broken position of the PDA slant culture medium with the broken culture surface, and after the treatment in the step, the infectious microbe pollution degree of re-germinated hyphae can be reduced, and the subsequent purification frequency is reduced. According to the method, the culture medium at the front end and the culture medium at the rear end of the inclined surface of the test tube are disconnected, the interval is 0.8-1.2 cm, mother strains to be inoculated taken from a flat plate are inoculated to the interval, inoculation blocks are removed after hyphae germ