PAPN mutant, composition for site-specific modification of pAPN gene and application of pAPN mutant

The invention provides a pAPN mutant, a composition for pAPN gene site-specific modification and application, and relates to the technical field of gene editing. The 734 site of the pAPN mutant is alanine, the mutated pAPN mutant can maintain self normal expression, meanwhile, the specific binding c...

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Bibliographische Detailangaben
Hauptverfasser:	HUANG LEI, LI KUI, MOU YULIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a pAPN mutant, a composition for pAPN gene site-specific modification and application, and relates to the technical field of gene editing. The 734 site of the pAPN mutant is alanine, the mutated pAPN mutant can maintain self normal expression, meanwhile, the specific binding capacity of a host expressing the pAPN mutant and TGEV is reduced, and the pAPN mutant has important scientific and practical significance in the aspect of pig disease resistance breeding. 本发明提供了一种pAPN突变体和用于pAPN基因定点修饰的组合物及应用，涉及基因编辑技术领域。该pAPN突变体的734位为丙氨酸，突变后的pAPN突变体能够维持自身正常表达，同时降低表达该pAPN突变体的宿主与TGEV发生特异性结合能力，在猪抗病育种方面具有重要的科学和实际意义。