Method for infecting meloidogyne by utilizing paecilomyces lilacinus and application

The invention discloses a method for infecting meloidogyne by utilizing paecilomyces lilacinus. The method comprises the following steps: S1, culturing paecilomyces lilacinus fermentation liquor; s2, culturing the root-knot nematode in vitro; and S3, infecting the root-knot nematodes by using paecil...

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Hauptverfasser: LAI QINGGUI, CHANG YOUMIN, HE WANGQUAN, XU LITING, CHEN JIAJIA, LIN ZHENYANG, LIU CHANGQING, ZHANG BIYAO, LI BINGHUA, LIN HUIJU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for infecting meloidogyne by utilizing paecilomyces lilacinus. The method comprises the following steps: S1, culturing paecilomyces lilacinus fermentation liquor; s2, culturing the root-knot nematode in vitro; and S3, infecting the root-knot nematodes by using paecilomyces lilacinus. According to the method for infecting the root-knot nematode by utilizing the paecilomyces lilacinus in the technical scheme, the operation process of an experiment is simplified, and the sterility of an experiment system is ensured; a large number of pure multi-stage root-knot nematodes can be obtained, and the effect of infecting the root-knot nematodes by the paecilomyces lilacinus can be tested more simply and accurately. The invention further discloses application of the paecilomyces lilacinus in prevention and treatment of root-knot nematodes of crops. 本发明公开了一种利用淡紫拟青霉侵染根结线虫的方法,包括以下几个步骤:S1培养淡紫拟青霉发酵液;S2体外培养根结线虫;S3使用淡紫拟青霉侵染所述根结线虫。采用以上技术方案的利用淡紫拟青霉侵染根结线虫的方法,简化了实验的操作过程,保证了实验体系无菌性;能够得到大量纯净的多阶段的根结线虫