Jujube tree stem tip detoxification method

The invention discloses a jujube tree stem tip detoxification method. The method comprises the steps of material pre-culture, secondary stem tip cutting, loading, vitrification ultra-low temperature treatment, unfreezing and recovery culture. According to the method, pre-culture is performed in the culture medium with an optimized formula, and the stem tips are cut for the second time, so that the survival rate of the stem tip explants is effectively increased, and the contradiction among the stem tip size, the survival rate and the detoxification rate is solved to a certain extent. The phytoplasma is detoxified through ultralow-temperature cryotherapy treatment, the survival rate of explants reaches 50%, and the detoxification rate is 100%. Therefore, the winter jujube stem tip ultralow-temperature freezing detoxification technology can effectively remove the phytoplasma of the winter jujube. 本发明公开了一种枣树茎尖脱毒的方法，包括材料预培养、二次切取茎尖、装载、玻璃化超低温处理、解冻、恢复培养的步骤。本发明通过优化配方的培养基中进行预培养，并二次切取茎尖，有效提高茎尖外植体的存活率，一定程度上解决了茎尖大小、成活率和脱毒