Internal reference gene for fluorescence quantification of stems of different parts of Chinese yam as well as primer and application of internal reference gene

The invention relates to a reference gene for fluorescence quantification of stems of different parts of Chinese yam as well as a primer and application of the reference gene, and belongs to the technical field of Chinese yam molecular biology. The nucleotide sequence of the reference gene MYB7 is as shown in SEQ ID NO. 1. The nucleotide sequence of a PCR (Polymerase Chain Reaction) amplification primer of the reference gene MYB7 is as follows: an upstream primer is as shown in SEQ ID NO. 2; and the downstream primer is as shown in SEQ ID NO.3. According to the invention, the reference genes which are relatively stable in stem expression at different parts of the Chinese yam are screened out, and a reference basis is provided for later research work such as gene function verification of growth and development and metabolic mechanism of the Chinese yam stem. 本发明涉及一种山药不同部位茎蔓荧光定量的内参基因及其引物与应用，属于薯蓣山药分子生物学技术领域。所述内参基因MYB7的核苷酸序列如SEQ ID NO.1所示。内参基因MYB7的PCR扩增引物核苷酸序列：上游引物如SEQ ID NO.2所示；下游引物如SEQ ID NO.3所示。本发明筛选出了在山药不同部位茎