Primer pair, kit thereof and application of primer pair in detection of pomacea canaliculata biomass in water body

The invention discloses a primer pair, a kit thereof and application of the primer pair and the kit in detection of pomacea spp. Biomass in a water body. DNA sequences of a qPCR (quantitative polymerase chain reaction) primer pair are shown as SEQ ID NO.5 and SEQ ID NO.6. The invention further discloses a kit for detecting pomacea spp. According to the method, the environment DNA technology is applied to invasion investigation and research, when the environment DNA in the water body is obtained, only water samples need to be collected within a certain range of the target water body, compared with a traditional method, appearance identification of a large number of species does not need to be carried out, and for a complex environment, the working difficulty is reduced, and a large number of manpower and financial resources are saved. Meanwhile, a mathematical model of the relation between eDNA of the ampullaria gigas with different densities and the Cq value of the qPCR reaction is successfully constructed, t