Anti-CD30ki-14 antibody as well as preparation method and application thereof

The invention relates to the field of antibodies, and particularly discloses an anti-CD30ki-1amp antibody and a preparation method thereof. 4 antibodies, and a preparation method and applications thereof. The antibody is anti-CD30ki-1amp; the CD30ki-1amp antibody comprises a light chain variable region and a heavy chain variable region which comprise specific sequences, and the CD30ki-1amp antibody is anti-CD30ki-1amp; the binding site of the antibody 1, 4 is a homologous fragment of a ki-1 subunit and a ki-4 subunit of CD30; the preparation method comprises the following steps: preparing CD30ki-1amp; 4, a universal antigen; carrying out animal immunization by using the universal antigen; establishing rabbit source antibody VH and VL gene banks by using spleen lymphocytes of the immunized rabbits; the method comprises the following steps: constructing an anti-CD30ki-1amp; 4, a rabbit chimeric human Fab antibody; constructing a rabbit chimeric human Fab antibody phage display library, and performing panning; a