IgM (Immunoglobulin M) functionalized bionic nano toxoid vaccine as well as preparation method and application thereof

The invention relates to the technical field of vaccines, in particular to an IgM (Immunoglobulin M) functionalized bionic nano toxoid vaccine as well as a preparation method and application thereof. The erythrocyte membrane-hybrid lipidosome toxoid vaccine can load bacterial pore-forming toxin, protein toxin does not need to be inactivated, the activity of the erythrocyte membrane-hybrid lipidosome toxoid vaccine is greatly enhanced, the immunogenicity is reserved to the maximum extent, further folic acid is used for modifying the nano vaccine, natural IgM can be adsorbed on the surface of a nano carrier in vivo, the antigen presentation efficiency is remarkably improved, and the immunogenicity of the erythrocyte membrane-hybrid lipidosome toxoid vaccine is improved. Immune response is enhanced, and a high-titer specific antibody is generated after immunization to prevent bacterial infection. 本发明涉及疫苗技术领域，具体是一种IgM功能化的仿生纳米类毒素疫苗及其制备方法和应用。本发明的红细胞膜-杂合脂质体类毒素疫苗可以装载细菌成孔毒素，无须灭活蛋白毒素，使其活性极大增强，最大限度保留免疫原性，进一步以叶酸对纳米疫苗进行修饰