Synchronous quantitative detection method for various metabolites in biological sample

The invention discloses a method for synchronously and quantitatively detecting various metabolites in a biological sample, which adopts chromatography-tandem mass spectrometry to detect the biological sample and comprises the following steps: (1) optimizing mass spectrometry detection parameters to obtain optimal mass spectrometry detection parameters of each metabolite; (2) detecting a biological sample by using the optimal mass spectrometric detection parameters, and classifying all metabolites; (3) optimizing and obtaining the final CE value of each metabolite; and (4) detecting the biological sample to be detected by adopting the final CE value corresponding to each metabolite, and quantitatively analyzing each metabolite in the biological sample to be detected by adopting an internal standard method to obtain the content of each metabolite in the biological sample to be detected. Meanwhile, the invention further provides an absolute quantitative analysis method based on elimination of natural isotope in