Primer probe composition for rapidly detecting wolf tooth virus through isothermal amplification and application of primer probe composition

The invention relates to the technical field of biology, particularly discloses a primer probe composition for rapidly detecting a wolf tooth virus through isothermal amplification and application of the primer probe composition, and further discloses a kit which contains the primer probe compositio...

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Bibliographische Detailangaben
Hauptverfasser: LIU JIA, DAI LIZHONG, DING PINGHUI, CAO CHANGZHENG, JI BOZHI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of biology, particularly discloses a primer probe composition for rapidly detecting a wolf tooth virus through isothermal amplification and application of the primer probe composition, and further discloses a kit which contains the primer probe composition and is used for rapidly detecting the wolf tooth virus through isothermal amplification. The sequences of a forward primer, a reverse primer and a probe in the primer and probe composition are nucleotide sequences shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively. The primer and probe composition is mainly applied to isothermal amplification rapid detection of the wolf tooth virus, an amplification system is fully freeze-dried, operation steps are greatly saved, the detection time is shortened, and the detection efficiency is improved. And moreover, the detection of the wolf tooth virus is completed through a multiple isothermal amplification detection mode based on recombinase, and the specificity a