Fluorescence labeling primer group of rice blast resistance gene Pigm and application of fluorescence labeling primer group

The invention discloses a fluorescence labeling primer group of a rice blast-resistant gene Pigm and application of the fluorescence labeling primer group, and relates to the technical field of genetic breeding. The fluorescence labeling primer group G-Pigm of the rice blast resistance gene Pigm disclosed by the invention is composed of a forward primer Pigm-F-G, a forward primer Pigm-F-T and a universal reverse primer Pigm-R. A PCR amplification product is detected by using a microplate reader to obtain a fluorescence signal, the Pigm genotype in a rice sample can be rapidly identified through conversion analysis, electrophoresis detection is not needed, the method is simple and reliable, the detection time is short, and the detection efficiency is high. The method is rapid in detection, is suitable for rapid screening of high-throughput samples, and can be used for directionally breeding new rice blast-resistant varieties containing Pigm genes. 本发明公开了水稻抗稻瘟病基因Pigm的荧光标记引物组及其应用，涉及遗传育种技术领域。本发明公开的水稻抗稻瘟病基因Pigm的荧光