Fermentation and purification process of MNR-MBP protein

The invention discloses construction of a strain for expressing MNR-MBP protein and a fusion protein fermentation and purification process. The method and the strain provided by the invention not only can reduce the degradation of the recombinant fusion protein, but also cannot generate obvious adve...

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Bibliographische Detailangaben
Hauptverfasser:	QI LILI, LIU MINGXIA, SONG XU, WANG GUANLIAN, LIU YI, FU YUJIE, CAI JIONG, CHEN YU, YANG XUE, MOU CHEN, XIAO KAI, DING GUOZHONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses construction of a strain for expressing MNR-MBP protein and a fusion protein fermentation and purification process. The method and the strain provided by the invention not only can reduce the degradation of the recombinant fusion protein, but also cannot generate obvious adverse effects on the biological activity of the recombinant fusion protein, and have important significance on improving the stability of a product using the recombinant fusion protein and reducing the unit dose cost. 本发明公开了表达MNR-MBP蛋白质的菌株构建和融合蛋白发酵和纯化工艺。本发明提供的方法和菌株既能够减少重组融合蛋白的降解、又不会对重组融合蛋白的生物活性产生显著不良影响，对于提高使用重组融合蛋白的产品稳定性、降低单位剂量成本具有重要意义。