D-amino acid oxidase, application thereof and method for preparing L-norvaline by enzyme method

The invention discloses D-amino acid oxidase, application thereof and a method for preparing L-norvaline by an enzyme method. The amino acid sequence of the D-amino acid oxidase is shown as SEQ ID NO. 1. The invention provides an optimized D-amino acid oxidase sequence for preparing L-norvaline by adopting an enzymatic method, and the D-amino acid oxidase has higher activity and substrate tolerance. The optimal conversion condition is determined by optimizing the experiment condition, and the conversion rate can be improved by the method, so that the production efficiency is improved; in addition, the method is small in pollution and more environment-friendly; meanwhile, raw materials are easy to obtain, the production cost can be reduced, and large-scale application is facilitated. 本发明公开了一种D-氨基酸氧化酶及其应用和酶法制备L-正缬氨酸的方法，该D-氨基酸氧化酶的氨基酸序列如SEQ ID NO.1所示。本发明为了采用酶法制备L-正缬氨酸，而提供了一种优化后的D-氨基酸氧化酶序列，该酶具有较高的活性和底物耐受度。并通过优化实验条件，确定最佳转化条件，通过该方法能够提高转化率，进而提高生产效率；并且使用该方法的污染小，对环境更友好；同时原料易得，不仅能够降低生产成本，还有利于大规模应用。