Production method for improving yield and therapeutic activity of extracellular vesicles

The invention discloses a production method for improving the yield and therapeutic activity of extracellular vesicles, which comprises the following steps: culturing cells capable of generating target extracellular vesicles in a cell culture medium containing an epidermal growth factor (EGF) and a tumor necrosis factor (TNF-alpha), providing a low-oxygen stimulation condition, collecting cell culture supernatant, and collecting the extracellular vesicles to obtain the extracellular vesicles. And obtaining the target extracellular vesicles. According to the method, triple stimulation of EGF, TNF-alpha and cell hypoxia induction is adopted, and the yield of the extracellular vesicles can be increased by promoting stem cell proliferation and promoting stem cells to secrete the extracellular vesicles at the same time; in addition, the yield-increased extracellular vesicles have an enhanced tissue damage treatment function. 本发明公开了一种提高细胞外囊泡产量、治疗活性的生产方法，该生产方法包括以下步骤：将能产生目标细胞外囊泡的细胞置于含表皮生长因子(EGF)、肿瘤坏死因子(TNF-α)的细胞培养基中培