Establishment method of immune response model and cell response test method

The invention belongs to the field of biology, and discloses a method for establishing a chicken TCR gamma delta + CD8 alpha + T cell immune response model, which comprises the following steps: step 1, taking lymphocytes of the spleen of a chicken infected with avian influenza virus, and inoculating the lymphocytes with the avian influenza virus to obtain antigen presenting cells; and 2, mixing lymphocytes and antigen presenting cells of the spleen of the chicken infected with the avian influenza virus, and culturing. The model established based on the method can well realize proliferation simulation of TCR gamma delta + CD8 alpha + T cells infected by the H9N2 subtype avian influenza viruses, and the model can accurately reflect the virulence of different strains of H9N2 subtype avian influenza viruses and the response ability of TCR gamma delta + CD8 alpha + T cells to different strains of H9N2 subtype avian influenza viruses. Meanwhile, the invention further discloses a chicken TCR gamma delta + CD8 alpha