Probe, primer and reagent for detecting bloodstream infection fungal pathogens by PCR-reverse dot blot hybridization technology and application of probe, primer and reagent

The invention provides a probe for detecting a bloodstream infection fungal pathogen by a PCR-reverse dot blot hybridization technology, which comprises thirteen amino-labeled specific hybridization probes, the sequences are shown as SEQ ID NO: 1-SEQ ID NO: 13, and the 5'end is labeled with ami...

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Bibliographische Detailangaben
Hauptverfasser:	LIN CHUNHUI, TANG HAO, XU HUAMING, ZHOU JING, HU XINYI, SHEN JILU, LIU RUNDE, JIANG TONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a probe for detecting a bloodstream infection fungal pathogen by a PCR-reverse dot blot hybridization technology, which comprises thirteen amino-labeled specific hybridization probes, the sequences are shown as SEQ ID NO: 1-SEQ ID NO: 13, and the 5'end is labeled with amino. The invention provides a primer for detecting a bloodstream infection fungal pathogen by a PCR-reverse dot blot hybridization technology, the primer comprises a universal-F and a universal-R, the sequences of the universal-F and the universal-R are respectively shown as SEQ ID NO: 16 and SEQ ID NO: 17, and the 5'ends of the universal-F and the universal-R are respectively marked with biotin. The invention also provides a reagent for detecting the bloodstream infected fungal pathogens by using the PCR-reverse dot blot hybridization technology and a method for detecting the fungal pathogens based on the PCR-reverse dot blot hybridization technology. According to the invention, rapid and accurate detection of thirteen