Probe, primer and reagent for detecting intestinal pathogens by multiple PCR-reverse dot blot hybridization technology and application of probe, primer and reagent

The invention provides a probe for detecting intestinal pathogens by a multiplex PCR (polymerase chain reaction)-reverse dot blot hybridization technology, which comprises thirteen amino-labeled specific hybridization probes, the base sequences of the probes are shown as SEQ ID NO: 1-SEQ ID NO: 13,...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIN CHUNHUI, TANG HAO, XU HUAMING, ZHOU JING, HU XINYI, SHEN JILU, LIU RUNDE, JIANG TONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides a probe for detecting intestinal pathogens by a multiplex PCR (polymerase chain reaction)-reverse dot blot hybridization technology, which comprises thirteen amino-labeled specific hybridization probes, the base sequences of the probes are shown as SEQ ID NO: 1-SEQ ID NO: 13, and the 5'end of each probe is labeled with amino. The invention provides primers for detecting intestinal pathogens by a multiplex PCR-reverse dot blot hybridization technology, the sequences of the primers are respectively shown as SEQ ID NO: 16-SEQ ID NO: 23, and biotin is marked at 5'ends. The invention further provides a reagent for detecting the intestinal pathogens based on the multiple PCR-reverse dot blot hybridization technology and a method for detecting the intestinal pathogens based on the multiple PCR-reverse dot blot hybridization technology. The method has the advantages of rapidness, convenience, low cost, strong adaptability and the like, and thirteen diarrhea pathogens can be simultaneously ident