Specific primer and kit for detecting marsh snail and application of specific primer and kit

The invention relates to a specific primer for detecting marsh snail, a kit and application, and belongs to the technical field of biological information. The method comprises the following steps: 1, sequencing to obtain target species sequence data, and constructing a sequence data set; 2, sequence alignment is carried out in an NCBI database; 3, analyzing and optimizing the compared sequence, and synthesizing a primer; and 4, on the basis of the primers, reagents required by PCR reaction are added, a PCR detection kit is constructed, and operation and application are convenient. The qPCR technology is utilized to realize signal amplification, the sensitivity is high, the specificity is good, and the specific detection of the marsh snail samples collected in the same region can be realized. 本发明涉及一种检测沼螺的特异性引物、试剂盒及应用，属于生物信息技术领域。本发明采取的措施包括：1、测序获取目标物种序列数据，构建序列数据集；2、在NCBI数据库中进行序列比对；3、将比对后的序列进行分析优化，合成引物；4、在引物基础上，添加PCR反应所需试剂，构建PCR检测试剂盒，方便操作应用。本发明利用qPCR技术实现信号扩增放大，灵敏度高、特异性好，可对同地区采集的沼螺样本实现特异性检测。