Preparation and application of serum-free mesenchymal stem cell culture medium

The invention provides preparation and application of a serum-free mesenchymal stem cell culture medium, and relates to the technical field of regenerative medicine. The preparation method of the serum-free mesenchymal stem cell culture medium comprises the following specific steps: S1, firstly, cleaning a workbench, an instrument and a tool to ensure that the workbench, the instrument and the tool are in a sterile environment; s2, preparing a basic culture medium; s3, adding a C-phycocyanin solution, and fully and uniformly dissolving the C-phycocyanin solution by slightly shaking or stirring; s4, gradually adding nutrient substances, growth factors and auxiliary agents; s5, fully mixing the culture medium, and adjusting the pH value to 7.2-7.4; s6, filtering the culture medium through a filter or an ultrafiltration device so as to remove suspended solids and bacteria and ensure the sterility of the culture medium; and S7, sterilizing and storing. According to the invention, the survival, proliferation, diff