Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus
The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax...
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creator | LIAO ZHENFENG LIANG YING QIANG BAOBAO WEI KUNHUA MIAO JIANHUA |
description | The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax notoginseng seeds germinate serve as explants, and the explants are induced through a culture medium to form the calluses; inducing somatic embryos of the calluses through a culture medium, and inducing germination through the culture medium; inducing buds formed by the somatic embryos into seedlings through a culture medium; a culture medium for inducing germination of the somatic embryos is MS + 4-12mg/L GA3. In one example, MS + 8mg/L GA3 is used for inducing germination of somatic embryos, and the germination rate can reach 52.86%. The invention provides a rapid propagation method from seeds to seedlings, the used explants have vigorous growth ability and differentiation ability, and the defect that materials a |
format | Patent |
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The method comprises the steps that stems or leaves of seedlings obtained after panax notoginseng seeds germinate serve as explants, and the explants are induced through a culture medium to form the calluses; inducing somatic embryos of the calluses through a culture medium, and inducing germination through the culture medium; inducing buds formed by the somatic embryos into seedlings through a culture medium; a culture medium for inducing germination of the somatic embryos is MS + 4-12mg/L GA3. In one example, MS + 8mg/L GA3 is used for inducing germination of somatic embryos, and the germination rate can reach 52.86%. The invention provides a rapid propagation method from seeds to seedlings, the used explants have vigorous growth ability and differentiation ability, and the defect that materials a</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230908&DB=EPODOC&CC=CN&NR=116711636A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,777,882,25545,76296</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230908&DB=EPODOC&CC=CN&NR=116711636A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIAO ZHENFENG</creatorcontrib><creatorcontrib>LIANG YING</creatorcontrib><creatorcontrib>QIANG BAOBAO</creatorcontrib><creatorcontrib>WEI KUNHUA</creatorcontrib><creatorcontrib>MIAO JIANHUA</creatorcontrib><title>Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus</title><description>The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax notoginseng seeds germinate serve as explants, and the explants are induced through a culture medium to form the calluses; inducing somatic embryos of the calluses through a culture medium, and inducing germination through the culture medium; inducing buds formed by the somatic embryos into seedlings through a culture medium; a culture medium for inducing germination of the somatic embryos is MS + 4-12mg/L GA3. In one example, MS + 8mg/L GA3 is used for inducing germination of somatic embryos, and the germination rate can reach 52.86%. The invention provides a rapid propagation method from seeds to seedlings, the used explants have vigorous growth ability and differentiation ability, and the defect that materials a</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi0EKwjAQRbtxIeodxgO4KIW6lqK40ZX7MibTNJBmQmYC9vZm4QFcfB483t829kE6s4WJM2RM3oYVUuaEDtVHB-pFCoEpQUsmECIbqhfQOXNxMySM-IHIys5HoXqh5Z1XdhS9AYMhFNk3mwmD0OHHXXO8XV_D_USJR5KEhiLpODzbtj_Xdf2l-6f5AojMQB8</recordid><startdate>20230908</startdate><enddate>20230908</enddate><creator>LIAO ZHENFENG</creator><creator>LIANG YING</creator><creator>QIANG BAOBAO</creator><creator>WEI KUNHUA</creator><creator>MIAO JIANHUA</creator><scope>EVB</scope></search><sort><creationdate>20230908</creationdate><title>Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus</title><author>LIAO ZHENFENG ; LIANG YING ; QIANG BAOBAO ; WEI KUNHUA ; MIAO JIANHUA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116711636A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>LIAO ZHENFENG</creatorcontrib><creatorcontrib>LIANG YING</creatorcontrib><creatorcontrib>QIANG BAOBAO</creatorcontrib><creatorcontrib>WEI KUNHUA</creatorcontrib><creatorcontrib>MIAO JIANHUA</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIAO ZHENFENG</au><au>LIANG YING</au><au>QIANG BAOBAO</au><au>WEI KUNHUA</au><au>MIAO JIANHUA</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus</title><date>2023-09-08</date><risdate>2023</risdate><abstract>The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax notoginseng seeds germinate serve as explants, and the explants are induced through a culture medium to form the calluses; inducing somatic embryos of the calluses through a culture medium, and inducing germination through the culture medium; inducing buds formed by the somatic embryos into seedlings through a culture medium; a culture medium for inducing germination of the somatic embryos is MS + 4-12mg/L GA3. In one example, MS + 8mg/L GA3 is used for inducing germination of somatic embryos, and the germination rate can reach 52.86%. The invention provides a rapid propagation method from seeds to seedlings, the used explants have vigorous growth ability and differentiation ability, and the defect that materials a</abstract><oa>free_for_read</oa></addata></record> |
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subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
title | Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus |
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