Method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic callus

The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax...

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Hauptverfasser: LIAO ZHENFENG, LIANG YING, QIANG BAOBAO, WEI KUNHUA, MIAO JIANHUA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for rapidly propagating tissue culture seedlings through panax notoginseng embryogenic calluses, and belongs to the technical field of panax notoginseng tissue culture propagation. The method comprises the steps that stems or leaves of seedlings obtained after panax notoginseng seeds germinate serve as explants, and the explants are induced through a culture medium to form the calluses; inducing somatic embryos of the calluses through a culture medium, and inducing germination through the culture medium; inducing buds formed by the somatic embryos into seedlings through a culture medium; a culture medium for inducing germination of the somatic embryos is MS + 4-12mg/L GA3. In one example, MS + 8mg/L GA3 is used for inducing germination of somatic embryos, and the germination rate can reach 52.86%. The invention provides a rapid propagation method from seeds to seedlings, the used explants have vigorous growth ability and differentiation ability, and the defect that materials a