Method and system for generating target FastQ file from undetermined FastQ files generated by NGS

The invention provides a method and system for generating a target FastQ file from an undetermined FastQ file generated by an NGS, a computer readable storage medium and a terminal. The method comprises the following steps: S1, preparing an original file and Index information to obtain an undetermined FastQ file generated by the NGS; s2, generating and correcting segmentation points; s3, decompressing, filtering and compressing; and S4, merging. The system comprises a database unit which is used for storing original data generated by an NGS, sample information and data generated after analysis; the processing unit comprises a segmentation point generating and correcting module, a decompression filtering compression module and a merging module; the database unit is in communication with the processing unit. According to the method and the device, the FastQ file matched with the barcode provided by the client can be quickly and accurately filtered from the undetermined FastQ file generated by the NGS, and the p