Identification method of salvianolic acid A target spot and application thereof

The invention belongs to the technical field of molecular biological detection of salvianolic acid A. The invention discloses a salvianolic acid A target identification method and application thereof. H9C2 cells are cultured and fused, salvianolic acid A is added into a neutral culture medium to incubate the cells for a certain time, total cell protein is extracted for cell thermal displacement analysis, then SDS-PAGE gel electrophoresis is performed, and the salvianolic acid A target identification method is obtained. Detecting the thermal degradation condition of the cell protein by utilizing coomassie brilliant blue staining; collecting protein bands, performing differential protein detection by using QE mass spectrometry, and determining that the GOT2 protein is the target of the salvianolic acid A; respectively detecting the thermal stability and pancreatin stability of the GOT2 protein under the condition that the salvianolic acid A is not treated; the method comprises the following steps: treating H9C2