Screening method and application of pancreatic cancer biomarker

The invention relates to the field of pancreatic cancer, in particular to a pancreatic cancer biomarker screening method and application thereof. The invention relates to a pancreatic cancer biomarker screening method, which comprises: taking out a blood sample, unfreezing, washing with PBS, adding ddH2O, homogenizing, swirling, adding a methanol and acetonitrile mixed solution, swirling, carrying out ultrasonication, centrifuging, and carrying out vacuum freeze drying on the supernatant, re-dissolving the freeze-dried powder, separating by adopting ultra-high performance liquid chromatography, and then detecting by adopting electrospray ionization mass spectrometry; after the detection is finished, identifying a molecular product through a mass spectrometer; carrying out primary screening on molecular products by adopting an OPLS-DA model to obtain VIPgt; 1 is a screening standard, and then screening the differential molecules obtained by primary screening again to obtain the pancreatic cancer biomarker. The