Lignin determination method suitable for tobacco raw material

The invention discloses a method suitable for measuring lignin in a tobacco raw material, which is used for measuring the content of the lignin in the tobacco raw material by an acetyl bromide method and mainly comprises the following steps: (1) grinding tobacco leaves or tobacco stems, drying by using a drying oven, weighing about 6.0 mg of tobacco leaf or tobacco stem powder, and putting into a 25ml glass tube with a stopper; (2) preparing a lignin standard solution; and (3) the optimal reaction conditions are obtained by optimizing the volume fraction of acetyl bromide, the reaction temperature, the reaction time, the addition amount of perchloric acid and the like. The method has the advantages that the steps are simple, the prepared lignin standard liquid can be used for one month, the operation is rapid, the determination is accurate, the repeatability is high, and the method is suitable for mass determination of samples. 本发明公开了一种适用于烟草原料木质素测定方法，通过乙酰溴法测定烟草原料木质素含量，主要通过以下步骤实现，(1)把烟叶或烟梗磨碎，用烘箱烘干，称取6.0mg左右的烟叶