Method and kit for detecting human T-lymphotropic I virus through real-time fluorescent loop-mediated isothermal amplification

The invention discloses a method and a kit for detecting human T-lymphotropic I virus through real-time fluorescent loop-mediated isothermal amplification. Specific oligonucleotide primers for real-time fluorescent loop-mediated isothermal amplification technology detection are respectively designed for a PX gene and an ACTB gene of HTLV-1 through professional software; a to-be-detected sample template is amplified by the primer sequence group, specific amplification of a target gene segment is carried out, and whether a sample contains the HTLV-1 virus is analyzed by judging whether two fluorescence amplification curves appear or not according to the added fluorescent dye. According to the method, the condition of electrophoresis pollution after amplification is finished can be avoided, HTLV-1 detection can be realized only through a fluorescence amplification curve, interpretation is convenient, and the specificity and sensitivity of detection are ensured. 本发明公开了一种实时荧光环介导恒温扩增检测人类嗜T淋巴细胞Ⅰ型病毒的方法及试剂盒，本发明通过对HTLV