Primer composition for detecting avian influenza and Newcastle disease virus based on MALDI-TOF mass spectrum detection technology and application thereof

The invention discloses a primer composition for detecting avian influenza and Newcastle disease viruses based on an MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrum detection technology and application of the primer composition. The invention firstly discloses th...

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Hauptverfasser:	SONG YUEQIAN, WANG LIN, ZHANG XIAOHAN, GAO ZHIQIANG, PU JING, REN TONG, GUO YOURAN, GUO HUIMIN, DU SILE, ZHANG JIAWEI, ZHAO XIANGPENG, LAI PING'AN, BAI ZILONG, ZHANG WEI, SHI XIJU, ZHONG YAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer composition for detecting avian influenza and Newcastle disease viruses based on an MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrum detection technology and application of the primer composition. The invention firstly discloses the primer composition for detecting the avian influenza virus and the Newcastle disease virus based on the MALDI-TOF mass spectrum detection technology. The primer composition comprises an amplification primer group as shown in SEQ ID No.1-16 and a UEP extension primer group as shown in SEQ ID No.17-31. The invention further discloses a kit for detecting the avian influenza virus and the Newcastle disease virus based on the MALDI-TOF mass spectrum detection technology. The invention further discloses a method for detecting the avian influenza virus and the Newcastle disease virus based on the MALDI-TOF mass spectrum detection technology. The MALDI-TOF mass spectrum detection method disclosed by the invention is sens