Method for rapid multiplication culture of TILs by using autologous tumor cells and culture and application

The invention belongs to the field of cell biology, and particularly relates to a method for rapid multiplication culture of TILs by using autologous tumor cells and cultures and application of the TILs. Specifically, according to the method disclosed by the invention, autologous tumor cells and supernate of tumor organoid cultured by the tumor cells are utilized to amplify autologous tumor infiltrating lymphocytes of a patient for three times, namely primary amplification, secondary amplification and tertiary amplification, so that a large number of tumor infiltrating lymphocytes are obtained in a relatively short time, and the activity of the tumor infiltrating lymphocytes is good. 本发明属于细胞生物学领域，具体涉及一种利用自体肿瘤细胞及培养物来快速扩增培养TILs的方法及应用。具体来说，本发明方法利用自体肿瘤细胞和用肿瘤细胞培养出的肿瘤类器官的上清液来分三次对患者自体肿瘤浸润淋巴细胞进行3次扩增，分别是初级扩增、二级扩增和三级扩增，在较短时间内获得数量较多的肿瘤浸润淋巴细胞，且活性好。