BMEC-released sEV, and preparation method, application and medicine thereof

The invention belongs to the technical field of medicines, and particularly relates to sEV released by BMEC, a preparation method and application thereof and a medicine. Adiponectin acts on cerebral microvascular endothelial cells to prepare the miR-31-5p expression down-regulated small extracellular vesicles, and the miR-31-5p expression down-regulated small extracellular vesicles have a better protection effect on cortical neuron apoptosis after cardiopulmonary resuscitation, so that the miR-31-5p expression down-regulated small extracellular vesicles have a good application prospect in preparation of drugs for relieving cortical neuron apoptosis after cardiopulmonary resuscitation. 本发明属于医药技术领域，具体涉及一种BMEC释放的sEV、其制备方法、用途及药物。本发明利用脂联素作用脑微血管内皮细胞，制备了miR-31-5p表达下调后的小细胞外囊泡，其对心肺复苏后皮质神经元凋亡具有更好的保护作用，因而其在制备用于减轻心肺复苏后皮质神经元凋亡的药物中具有很好的应用前景。