Large-scale dynamic and static online cell transfection system and application

The invention relates to a large-scale dynamic and static online cell transfection system and application, the system comprises a plasmid compound container, a transfection reagent compound container and a reactor, and also comprises an efficient quasi-dynamic transfection pipeline; the double-channel pumping system is arranged on the first pipeline and the second pipeline; wherein a mixed filler module is arranged in the transfection compound dynamic mixing tube and is used for performing turbulent flow mixing on a plasmid compound and a transfection reagent compound to form a transfection compound, an included angle is formed between the transfection compound quasi-static incubation tube and the transfection compound dynamic mixing tube, and the transfection compound quasi-static incubation tube and the transfection compound dynamic mixing tube incline upwards; and the transfection compound flows into the reactor after being incubated for a specific time to be combined with the cells and complete cell trans