Monoclonal nano antibody for recognizing monkey pox virus core protein A29L, application and monoclonal nano antibody library

The invention discloses a monoclonal nano antibody for recognizing monkey pox virus core protein A29L, application and a monoclonal nano antibody library, and relates to the technical field of monkey pox virus monoclonal antibody preparation. The number of the monoclonal nano antibodies provided by the invention is five, namely A6, B8, G9, H9 and E12, and the amino acid sequences of heavy chain complementary determining regions CDR1, CDR2 and CDR3 of the five monoclonal nano antibodies are respectively shown as SEQ ID NO.1-15. The invention also provides a monoclonal nano antibody library for screening and obtaining the monoclonal nano antibody as well as a construction method and an application method of the library. The nano antibody provided by the invention has very high affinity and specificity to monkey pox virus core protein A29L, and is strong in thermal stability. 本发明公开了一种识别猴痘病毒核心蛋白A29L的单克隆纳米抗体、应用及单克隆纳米抗体文库，涉及猴痘病毒单克隆抗体制备技术领域。本发明提供的单克隆纳米抗体为A6、B8、G9、H9、E12共5条，这5条单克隆纳米抗体重链互补决定区CDR1、CDR2、CDR3的氨基酸序列分别如SEQ