Recombinant protein subunit nasal mucosa vaccine aiming at new coronavirus infection as well as preparation method and application of recombinant protein subunit nasal mucosa vaccine

The invention belongs to the technical field of biological products, and particularly relates to a recombinant protein subunit nasal mucosa vaccine aiming at new coronavirus infection as well as a preparation method and application of the recombinant protein subunit nasal mucosa vaccine. The nasal mucosa protein subunit vaccine CS/S-RBD/O-HTCC is prepared by taking two polysaccharide derivatives of 6-O-quaternized chitosan (O-HTCC) and sulphated curdlan polysaccharide (CS) as mucosa adjuvants and taking an SARS-CoV-2 spike protein receptor binding domain S-RBD as an antigen. Experimental results show that the vaccine can induce BALB/c mice to generate effective systemic and local immune responses. In addition, the research finds that the booster immunity can generate a protection effect on a wild type S-RBD, a beta mutant type S-RBD and an omicro mutant type S-RBD. The result shows that the vaccine has a broad-spectrum protection effect, and has a huge application value as a new coronavirus vaccine reinforcem