Method for creating new germplasm through hybridization and cesium radiation of macadamia nuts

The invention discloses a method for creating new germplasm through hybridization and cesium radiation of macadamia nuts. The method comprises the following steps: (1) male parent selection; (2) pretreatment of male parents of macadamia nuts; (3) pretreatment of male parent pollen; (4) mutagenesis t...

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Bibliographische Detailangaben
Hauptverfasser: HUANG XIYUN, HE XIANYANG, YANG XIAOZHOU, SONG HAIYUN, XU PENG, TAN QIUJIN, ZHOU CHUNHENG, ZHENG SHUFANG, WEI YUANRONG, WANG WENLIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for creating new germplasm through hybridization and cesium radiation of macadamia nuts. The method comprises the following steps: (1) male parent selection; (2) pretreatment of male parents of macadamia nuts; (3) pretreatment of male parent pollen; (4) mutagenesis treatment of caesium-137; (5) pollination; and (6) harvesting selected products. The method comprises the following steps: firstly, selecting a macadamia nut male parent variety of which the pollinated flower number, the fruiting rate, the fruit setting rate and other data are far higher than those of a control group, then carrying out three-stage illumination control and moisture control on the macadamia nut male parent, spraying a pretreatment solution, and meanwhile, carrying out pretreatment on male parent pollen, so that the pollen can adapt to optical radiation in advance, and the adaptability of the pollen to caesium-137 ray irradiation is enhanced; by adopting the method, the sensitivity of pollen to caesium