Electrochemical sensor as well as preparation method and application thereof

The invention belongs to the technical field of electrochemical detection, and particularly provides an electrochemical sensor which comprises a working electrode, and the working electrode comprises a carbon fiber microelectrode and nanoparticles deposited on the surface of the carbon fiber microelectrode; the nano particles comprise nano ruthenium particles and nano gold particles. The electrochemical sensor has excellent sensitivity, stability and anti-interference performance in detection of ACOP, PAP and PNP by utilizing the synergistic electrocatalysis effect of the bimetallic nanoparticles. The electrochemical sensor can be successfully applied to electrochemical detection of human serum sample ACOP, the recovery rate reaches 95.9-100.5%, a solution is expected to be provided for quantitative analysis of ACOP, and the electrochemical sensor has huge application potential in pharmacodynamics and pharmacology research. The invention further provides a method for simultaneously or independently detecting