Primer and method for identifying mating type of boletus aereus YL1701-2 strain monokaryon

The invention provides a primer and a method for identifying the mating type of a boletus aereus YL1701-2 strain monokaryon, relates to the technical field of biology, and can rapidly and accurately identify the mating type of the YL1701-2 strain monokaryon so as to improve the working efficiency of crossbreeding based on the YL1701-2 strain. The primer comprises one or more pairs of a first primer pair, a second primer pair, a third primer pair, a fourth primer pair, a fifth primer pair, a sixth primer pair, a seventh primer pair and an eighth primer pair, wherein the eight pairs of primers respectively have sequences shown as SEQ ID NO.1 to SEQ ID NO.16 in a sequence table. According to the invention, PCR amplification is mainly carried out on a genome DNA template of a to-be-detected monokaryon by using the eight pairs of primers or a part of the primers, and genotypes of the A and B sites of the mating type gene are judged, so that the mating type of the monokaryon is judged. 本发明提供了一种鉴定黑牛肝菌YL1701-2菌株单核体交配