Gene for controlling formation and development of tobacco petioles as well as cloning method and application of gene

The invention discloses a gene for controlling tobacco petiole formation and development and a cloning method and application thereof, the gene for controlling tobacco petiole formation and development comprises an NtabKNOX1 gene, a parent of the NtabKNOX1 gene is selected from a flue-cured tobacco variety K326 and an aromatic tobacco germplasm Samsun, the NtabKNOX1 gene comprises a mutant Samsun-cas9-30 or Samsun-cas9-34, and the mutant Samsun-cas9-30 or Samsun-cas9-34 is selected from a mutant Samsun-cas9-30 or Samsun-cas9-34. According to the mutant Samsun-cas9-30, a T basic group is inserted into the first exon of the NtabKNOX1 gene, and an A basic group is inserted into the first exon of the NtabKNOX1 gene, and the mutant Samsun-cas9-34, the first exon of the NtabKNOX1 gene and the second exon of the NtabKNOX1 gene are respectively provided with an A basic group. The gene interval position is accurately positioned, the gene for controlling the growth and development of the tobacco petiole is cloned, the