Mammal early pregnancy detection marker and application thereof

The invention discloses a protein marker for early pregnancy detection of mammals, and particularly relates to a hidden marker protein serum amyloid A of early pregnancy test paper. According to the method, a liquid chromatography-mass spectrometry (LC-MS) technology is adopted for proteome sequencing, and sample correlation is compared through Venn, sample correlation, PCA and PLS-DA analysis; carrying out protein function annotation by adopting GO, KEGG, COG and Pfam, and carrying out positioning prediction on subcells; differential proteins, protein sets and differential metabolites are calculated and counted; association analysis of proteome and transcriptome is carried out to obtain early-stage pregnant sheep serum differential protein, and a key marker protein for early-stage sheep pregnancy is screened out, so that a basis is provided for early-stage sheep pregnancy detection of unknown sheep. 本发明公开了哺乳动物早期妊娠检测的蛋白标志物，尤其涉及早期妊娠试纸隐藏标志蛋白血清淀粉样物质A。本发明使用的蛋白组测序采用液相色谱-质谱(LC-MS)技术，通过Venn、样本相关性、PCA、PLS-DA分析对样品相关性进