Colloidal gold preparation method for test strip for detecting Helicobacter pylori IgG antibody and preparation method of test strip

The invention relates to a preparation method of colloidal gold for a helicobacter pylori IgG antibody detection test strip and a preparation method of the test strip, and relates to the technical field of biological immunization, and the preparation method of the colloidal gold for the helicobacter...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG YANAN, LIANG LITIAN, YANG FAN, LI CUNYAN, WANG CAIYUN, ZHANG JINGJING, YANG MINGXIA
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a preparation method of colloidal gold for a helicobacter pylori IgG antibody detection test strip and a preparation method of the test strip, and relates to the technical field of biological immunization, and the preparation method of the colloidal gold for the helicobacter pylori IgG antibody detection test strip comprises the following steps: (1) adding 100mL of 0.02% HAuCl4 solution into a three-neck flask, and stirring uniformly; placing the three-necked flask on a magnetic stirring electric jacket, condensing, refluxing and heating until boiling, stirring at the rotating speed of 300rpm, rapidly adding 2mL of 2% sodium citrate solution, reacting, continuously condensing, refluxing and heating for 10-15 minutes to generate a seed crystal solution, and cooling for later use, wherein the solution in the three-necked flask is changed from yellow to colorless to black and then gradually becomes pink; according to the preparation method, the problems of non-uniform particle morphology