Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus
The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. Ac...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | XU LU ZHU YUANYUAN LI FANGTAO PENG GUORUI ZOU XINGQI ZUO XUEZHI LIU YEBING ZHAO JUNJIE ZHAO QIZU XIA YINGJU XU XIAO'AI |
| description | The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. According to the detection kit capable of rapidly identifying the wild strain and the gene-deleted strain of the African swine fever virus, aiming at the gene-deleted strain of the African swine fever researched and developed in China at present, the gene sequences of B646L, EP402R, MGF505-3R and A137R of the African swine fever are selected to design and synthesize specific primers and probes aiming at the four genes; a method for identifying the wild strain and the gene-deleted strain of the African swine fever virus is established, a kit convenient for diagnosis is developed, and the kit has the characteristics of high sensitivity, strong specificity, good repeatability and good stability.
本发明属于分子生物学技术领域，具体涉及一种用于鉴 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116656874A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116656874A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116656874A3</originalsourceid><addsrcrecordid>eNqNjjELwjAUhLs4iPofnrsORa2upShOguBeYvOiD9u8mLxU3P3hRtDd6eDu4-6G2esYlfbRtQj3qKyQKKEewbSRPYYGrYDz1KFPwmeEhjvHgYTYzuBGAspqIJ04MtSojw8dypU1GPa_5En2AqXxibAQHmTTAvaptCcfwzgbGNUGnHx1lE1321O1n6PjGoNT6QZKXR3yvChWxWa9LBf_MG96P0w4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus</title><source>esp@cenet</source><creator>XU LU ; ZHU YUANYUAN ; LI FANGTAO ; PENG GUORUI ; ZOU XINGQI ; ZUO XUEZHI ; LIU YEBING ; ZHAO JUNJIE ; ZHAO QIZU ; XIA YINGJU ; XU XIAO'AI</creator><creatorcontrib>XU LU ; ZHU YUANYUAN ; LI FANGTAO ; PENG GUORUI ; ZOU XINGQI ; ZUO XUEZHI ; LIU YEBING ; ZHAO JUNJIE ; ZHAO QIZU ; XIA YINGJU ; XU XIAO'AI</creatorcontrib><description>The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. According to the detection kit capable of rapidly identifying the wild strain and the gene-deleted strain of the African swine fever virus, aiming at the gene-deleted strain of the African swine fever researched and developed in China at present, the gene sequences of B646L, EP402R, MGF505-3R and A137R of the African swine fever are selected to design and synthesize specific primers and probes aiming at the four genes; a method for identifying the wild strain and the gene-deleted strain of the African swine fever virus is established, a kit convenient for diagnosis is developed, and the kit has the characteristics of high sensitivity, strong specificity, good repeatability and good stability.
本发明属于分子生物学技术领域，具体涉及一种用于鉴</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230829&DB=EPODOC&CC=CN&NR=116656874A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230829&DB=EPODOC&CC=CN&NR=116656874A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XU LU</creatorcontrib><creatorcontrib>ZHU YUANYUAN</creatorcontrib><creatorcontrib>LI FANGTAO</creatorcontrib><creatorcontrib>PENG GUORUI</creatorcontrib><creatorcontrib>ZOU XINGQI</creatorcontrib><creatorcontrib>ZUO XUEZHI</creatorcontrib><creatorcontrib>LIU YEBING</creatorcontrib><creatorcontrib>ZHAO JUNJIE</creatorcontrib><creatorcontrib>ZHAO QIZU</creatorcontrib><creatorcontrib>XIA YINGJU</creatorcontrib><creatorcontrib>XU XIAO'AI</creatorcontrib><title>Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus</title><description>The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. According to the detection kit capable of rapidly identifying the wild strain and the gene-deleted strain of the African swine fever virus, aiming at the gene-deleted strain of the African swine fever researched and developed in China at present, the gene sequences of B646L, EP402R, MGF505-3R and A137R of the African swine fever are selected to design and synthesize specific primers and probes aiming at the four genes; a method for identifying the wild strain and the gene-deleted strain of the African swine fever virus is established, a kit convenient for diagnosis is developed, and the kit has the characteristics of high sensitivity, strong specificity, good repeatability and good stability.
本发明属于分子生物学技术领域，具体涉及一种用于鉴</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjjELwjAUhLs4iPofnrsORa2upShOguBeYvOiD9u8mLxU3P3hRtDd6eDu4-6G2esYlfbRtQj3qKyQKKEewbSRPYYGrYDz1KFPwmeEhjvHgYTYzuBGAspqIJ04MtSojw8dypU1GPa_5En2AqXxibAQHmTTAvaptCcfwzgbGNUGnHx1lE1321O1n6PjGoNT6QZKXR3yvChWxWa9LBf_MG96P0w4</recordid><startdate>20230829</startdate><enddate>20230829</enddate><creator>XU LU</creator><creator>ZHU YUANYUAN</creator><creator>LI FANGTAO</creator><creator>PENG GUORUI</creator><creator>ZOU XINGQI</creator><creator>ZUO XUEZHI</creator><creator>LIU YEBING</creator><creator>ZHAO JUNJIE</creator><creator>ZHAO QIZU</creator><creator>XIA YINGJU</creator><creator>XU XIAO'AI</creator><scope>EVB</scope></search><sort><creationdate>20230829</creationdate><title>Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus</title><author>XU LU ; ZHU YUANYUAN ; LI FANGTAO ; PENG GUORUI ; ZOU XINGQI ; ZUO XUEZHI ; LIU YEBING ; ZHAO JUNJIE ; ZHAO QIZU ; XIA YINGJU ; XU XIAO'AI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116656874A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>XU LU</creatorcontrib><creatorcontrib>ZHU YUANYUAN</creatorcontrib><creatorcontrib>LI FANGTAO</creatorcontrib><creatorcontrib>PENG GUORUI</creatorcontrib><creatorcontrib>ZOU XINGQI</creatorcontrib><creatorcontrib>ZUO XUEZHI</creatorcontrib><creatorcontrib>LIU YEBING</creatorcontrib><creatorcontrib>ZHAO JUNJIE</creatorcontrib><creatorcontrib>ZHAO QIZU</creatorcontrib><creatorcontrib>XIA YINGJU</creatorcontrib><creatorcontrib>XU XIAO'AI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>XU LU</au><au>ZHU YUANYUAN</au><au>LI FANGTAO</au><au>PENG GUORUI</au><au>ZOU XINGQI</au><au>ZUO XUEZHI</au><au>LIU YEBING</au><au>ZHAO JUNJIE</au><au>ZHAO QIZU</au><au>XIA YINGJU</au><au>XU XIAO'AI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus</title><date>2023-08-29</date><risdate>2023</risdate><abstract>The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. According to the detection kit capable of rapidly identifying the wild strain and the gene-deleted strain of the African swine fever virus, aiming at the gene-deleted strain of the African swine fever researched and developed in China at present, the gene sequences of B646L, EP402R, MGF505-3R and A137R of the African swine fever are selected to design and synthesize specific primers and probes aiming at the four genes; a method for identifying the wild strain and the gene-deleted strain of the African swine fever virus is established, a kit convenient for diagnosis is developed, and the kit has the characteristics of high sensitivity, strong specificity, good repeatability and good stability.
本发明属于分子生物学技术领域，具体涉及一种用于鉴</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116656874A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A54%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=XU%20LU&rft.date=2023-08-29&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116656874A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |