Quadruple quantitative fluorescent primer probe composition, kit and identification method for identifying African swine fever virus

The invention belongs to the technical field of molecular biology, and particularly relates to a quadruple fluorescent quantitative primer probe composition, a kit and a PCR (Polymerase Chain Reaction) identification method for identifying wild African swine fever virus and a gene-deleted strain. According to the detection kit capable of rapidly identifying the wild strain and the gene-deleted strain of the African swine fever virus, aiming at the gene-deleted strain of the African swine fever researched and developed in China at present, the gene sequences of B646L, EP402R, MGF505-3R and A137R of the African swine fever are selected to design and synthesize specific primers and probes aiming at the four genes; a method for identifying the wild strain and the gene-deleted strain of the African swine fever virus is established, a kit convenient for diagnosis is developed, and the kit has the characteristics of high sensitivity, strong specificity, good repeatability and good stability. 本发明属于分子生物学技术领域，具体涉及一种用于鉴