Method for improving bacterial wilt resistance of tobacco plants and application

The method for improving the bacterial wilt resistance of the tobacco plant on the first aspect comprises the following steps that NtCCoAOMT6 genes, NtCCoAOMT6L genes and Nt4CL genes in tobacco are knocked out in a fixed point mode through a CRISPR/CAS9 gene editing system, the bacterial wilt resist...

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Bibliographische Detailangaben
Hauptverfasser:	WANG JIN, CHEN JIANHUA, ZHANG CHENGMING, YANG GUANGYU, ZHU LONG, HU WEIYAO, LE ZHIWEI, ZHANG JIANDUO, KONG WEISONG, XU YONG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The method for improving the bacterial wilt resistance of the tobacco plant on the first aspect comprises the following steps that NtCCoAOMT6 genes, NtCCoAOMT6L genes and Nt4CL genes in tobacco are knocked out in a fixed point mode through a CRISPR/CAS9 gene editing system, the bacterial wilt resistance tobacco plant with the genes edited is obtained, and the sequences of the NtCCoAOMT6 genes, the sequences of the NtCCoAOMT6L genes and the sequences of the Nt4CL genes are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively. The invention further provides application of the method in the first aspect to improvement of agronomic traits of tobacco. The plant height, effective leaves, leaf width and leaf length of the gene-edited bacterial wilt-resistant tobacco plant are higher than those of the gene-unedited tobacco plant. The tobacco germplasm obtained by the invention has better resistance to tobacco bacterial wilt. The agricultural characters such as plant height, effective leaves, leaf width, lea