Preparation method of poxvirus vector vaccine

The invention provides a preparation method of a poxvirus vector vaccine. The preparation method comprises the following steps: S1, preparing a primary chicken embryo cell suspension; s2, culturing poxvirus; injecting a serum-free culture medium containing 0.1-0.5% of human serum albumin and 0.1-1.0 mmol/L of glutamine dipeptide into a disposable reaction bag, adding the cell suspension, and uniformly mixing; adding a virus infection enhancer into the serum-free culture medium, adding the virus suspension, uniformly mixing, standing at 4 DEG C, adding the virus suspension into the reaction bag, uniformly mixing and culturing; and S3, harvesting poxvirus. According to the preparation method, one-time reactor culture is adopted, the system takes a flaky carrier as a support of primary chick embryo cells, sterilization is not needed, the operation is simple and convenient, and the yield is high; the virus infectivity is increased by adding the virus proliferation agent, and the virus inoculation amount is reduce