Preparation method and application of sheep placenta exosome

The invention discloses a preparation method and application of a sheep placenta exosome. In combination with the structural characteristics of the placenta, other impurity components in sheep placenta tissues are effectively removed through physical methods of slicing, enzymolysis, microfiltration, ultrafiltration, freeze-drying and the like, and finally a sterile product rich in high-concentration exosomes is obtained. The method is high in sheep placenta exosome extraction efficiency and stable in process. The sheep placenta exosome prepared by the invention has good integrity and biological activity, and has the efficacy of promoting human skin fibroblasts to resist ultraviolet damage and repair within a certain concentration range. 本发明公开了一种羊胎盘外泌体的制备方法及应用。本发明结合胎盘结构特点，通过切片、酶解、微滤、超滤、冻干等物理学方法，有效的去除了羊胎盘组织中其他杂质成分，最终获得了富含高浓度外泌体无菌产物。该方法对羊胎盘外泌体的提取效率高，且工艺稳定。本发明制得的羊胎盘外泌体具有良好的完整性和生物学活性，在一定的浓度范围内，具有促进人皮肤成纤维细胞抗紫外损伤及修复的功效。