Tissue culture and rapid propagation method for agapanthus
The invention discloses a tissue culture and rapid propagation method for agapanthus, and belongs to a biological tissue culture technology. The method comprises the steps of explant obtaining, cleaning, disinfection, primary cluster bud induction, subculture cluster bud proliferation, cluster bud r...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | YANG SHENGLI TANG CHAOZAI ZI SHUHONG ZENG XIANGFEI ZI YUNWEI LI XIONGGANG ZI YIHONG ZI YINGJIE ZHAO LUQIN HE HOUGU YANG CHAOMEI |
| description | The invention discloses a tissue culture and rapid propagation method for agapanthus, and belongs to a biological tissue culture technology. The method comprises the steps of explant obtaining, cleaning, disinfection, primary cluster bud induction, subculture cluster bud proliferation, cluster bud rooting culture and domestication. Wherein an induction culture medium of the primary cluster buds is prepared from MS (Murashige and Skoog), NAA (Naphthalene Acetic Acid) of 0.02 to 0.05 mg/L, IAA (Indoleacetic Acid) of 0.01 to 0.02 mg/L, ZT of 0.5 to 0.8 mg/L and 2-ip of 0.1 to 0.2 mg/L; a proliferation culture medium of the subculture cluster buds is prepared from improved MS, 0.05 mg/L to 0.08 mg/L of IAA, 1.2 mg/L to 1.5 mg/L of ZT and 0.03 mg/L to 0.05 mg/L of BR; a rooting culture medium of the cluster buds is prepared from 1/2MS, 0.2-0.3 mg/L of NAA, 0.5-0.8 mg/L of IAA and 50-60ml/L of coconut juice. According to the method, the disinfection pollution rate, the disinfection death rate, the primary cluster b |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116649221A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116649221A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116649221A3</originalsourceid><addsrcrecordid>eNrjZLAKySwuLk1VSC7NKSktSlVIzEtRKEosyExRKCjKL0hMTyzJzM9TyE0tychPUUjLL1IAChUk5pVklBbzMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oLE5NS81JJ4Zz9DQzMzE0sjI0NHY2LUAACbcC9d</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Tissue culture and rapid propagation method for agapanthus</title><source>esp@cenet</source><creator>YANG SHENGLI ; TANG CHAOZAI ; ZI SHUHONG ; ZENG XIANGFEI ; ZI YUNWEI ; LI XIONGGANG ; ZI YIHONG ; ZI YINGJIE ; ZHAO LUQIN ; HE HOUGU ; YANG CHAOMEI</creator><creatorcontrib>YANG SHENGLI ; TANG CHAOZAI ; ZI SHUHONG ; ZENG XIANGFEI ; ZI YUNWEI ; LI XIONGGANG ; ZI YIHONG ; ZI YINGJIE ; ZHAO LUQIN ; HE HOUGU ; YANG CHAOMEI</creatorcontrib><description>The invention discloses a tissue culture and rapid propagation method for agapanthus, and belongs to a biological tissue culture technology. The method comprises the steps of explant obtaining, cleaning, disinfection, primary cluster bud induction, subculture cluster bud proliferation, cluster bud rooting culture and domestication. Wherein an induction culture medium of the primary cluster buds is prepared from MS (Murashige and Skoog), NAA (Naphthalene Acetic Acid) of 0.02 to 0.05 mg/L, IAA (Indoleacetic Acid) of 0.01 to 0.02 mg/L, ZT of 0.5 to 0.8 mg/L and 2-ip of 0.1 to 0.2 mg/L; a proliferation culture medium of the subculture cluster buds is prepared from improved MS, 0.05 mg/L to 0.08 mg/L of IAA, 1.2 mg/L to 1.5 mg/L of ZT and 0.03 mg/L to 0.05 mg/L of BR; a rooting culture medium of the cluster buds is prepared from 1/2MS, 0.2-0.3 mg/L of NAA, 0.5-0.8 mg/L of IAA and 50-60ml/L of coconut juice. According to the method, the disinfection pollution rate, the disinfection death rate, the primary cluster b</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230829&DB=EPODOC&CC=CN&NR=116649221A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230829&DB=EPODOC&CC=CN&NR=116649221A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YANG SHENGLI</creatorcontrib><creatorcontrib>TANG CHAOZAI</creatorcontrib><creatorcontrib>ZI SHUHONG</creatorcontrib><creatorcontrib>ZENG XIANGFEI</creatorcontrib><creatorcontrib>ZI YUNWEI</creatorcontrib><creatorcontrib>LI XIONGGANG</creatorcontrib><creatorcontrib>ZI YIHONG</creatorcontrib><creatorcontrib>ZI YINGJIE</creatorcontrib><creatorcontrib>ZHAO LUQIN</creatorcontrib><creatorcontrib>HE HOUGU</creatorcontrib><creatorcontrib>YANG CHAOMEI</creatorcontrib><title>Tissue culture and rapid propagation method for agapanthus</title><description>The invention discloses a tissue culture and rapid propagation method for agapanthus, and belongs to a biological tissue culture technology. The method comprises the steps of explant obtaining, cleaning, disinfection, primary cluster bud induction, subculture cluster bud proliferation, cluster bud rooting culture and domestication. Wherein an induction culture medium of the primary cluster buds is prepared from MS (Murashige and Skoog), NAA (Naphthalene Acetic Acid) of 0.02 to 0.05 mg/L, IAA (Indoleacetic Acid) of 0.01 to 0.02 mg/L, ZT of 0.5 to 0.8 mg/L and 2-ip of 0.1 to 0.2 mg/L; a proliferation culture medium of the subculture cluster buds is prepared from improved MS, 0.05 mg/L to 0.08 mg/L of IAA, 1.2 mg/L to 1.5 mg/L of ZT and 0.03 mg/L to 0.05 mg/L of BR; a rooting culture medium of the cluster buds is prepared from 1/2MS, 0.2-0.3 mg/L of NAA, 0.5-0.8 mg/L of IAA and 50-60ml/L of coconut juice. According to the method, the disinfection pollution rate, the disinfection death rate, the primary cluster b</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLAKySwuLk1VSC7NKSktSlVIzEtRKEosyExRKCjKL0hMTyzJzM9TyE0tychPUUjLL1IAChUk5pVklBbzMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oLE5NS81JJ4Zz9DQzMzE0sjI0NHY2LUAACbcC9d</recordid><startdate>20230829</startdate><enddate>20230829</enddate><creator>YANG SHENGLI</creator><creator>TANG CHAOZAI</creator><creator>ZI SHUHONG</creator><creator>ZENG XIANGFEI</creator><creator>ZI YUNWEI</creator><creator>LI XIONGGANG</creator><creator>ZI YIHONG</creator><creator>ZI YINGJIE</creator><creator>ZHAO LUQIN</creator><creator>HE HOUGU</creator><creator>YANG CHAOMEI</creator><scope>EVB</scope></search><sort><creationdate>20230829</creationdate><title>Tissue culture and rapid propagation method for agapanthus</title><author>YANG SHENGLI ; TANG CHAOZAI ; ZI SHUHONG ; ZENG XIANGFEI ; ZI YUNWEI ; LI XIONGGANG ; ZI YIHONG ; ZI YINGJIE ; ZHAO LUQIN ; HE HOUGU ; YANG CHAOMEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116649221A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>YANG SHENGLI</creatorcontrib><creatorcontrib>TANG CHAOZAI</creatorcontrib><creatorcontrib>ZI SHUHONG</creatorcontrib><creatorcontrib>ZENG XIANGFEI</creatorcontrib><creatorcontrib>ZI YUNWEI</creatorcontrib><creatorcontrib>LI XIONGGANG</creatorcontrib><creatorcontrib>ZI YIHONG</creatorcontrib><creatorcontrib>ZI YINGJIE</creatorcontrib><creatorcontrib>ZHAO LUQIN</creatorcontrib><creatorcontrib>HE HOUGU</creatorcontrib><creatorcontrib>YANG CHAOMEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YANG SHENGLI</au><au>TANG CHAOZAI</au><au>ZI SHUHONG</au><au>ZENG XIANGFEI</au><au>ZI YUNWEI</au><au>LI XIONGGANG</au><au>ZI YIHONG</au><au>ZI YINGJIE</au><au>ZHAO LUQIN</au><au>HE HOUGU</au><au>YANG CHAOMEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Tissue culture and rapid propagation method for agapanthus</title><date>2023-08-29</date><risdate>2023</risdate><abstract>The invention discloses a tissue culture and rapid propagation method for agapanthus, and belongs to a biological tissue culture technology. The method comprises the steps of explant obtaining, cleaning, disinfection, primary cluster bud induction, subculture cluster bud proliferation, cluster bud rooting culture and domestication. Wherein an induction culture medium of the primary cluster buds is prepared from MS (Murashige and Skoog), NAA (Naphthalene Acetic Acid) of 0.02 to 0.05 mg/L, IAA (Indoleacetic Acid) of 0.01 to 0.02 mg/L, ZT of 0.5 to 0.8 mg/L and 2-ip of 0.1 to 0.2 mg/L; a proliferation culture medium of the subculture cluster buds is prepared from improved MS, 0.05 mg/L to 0.08 mg/L of IAA, 1.2 mg/L to 1.5 mg/L of ZT and 0.03 mg/L to 0.05 mg/L of BR; a rooting culture medium of the cluster buds is prepared from 1/2MS, 0.2-0.3 mg/L of NAA, 0.5-0.8 mg/L of IAA and 50-60ml/L of coconut juice. According to the method, the disinfection pollution rate, the disinfection death rate, the primary cluster b</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116649221A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Tissue culture and rapid propagation method for agapanthus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T05%3A42%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=YANG%20SHENGLI&rft.date=2023-08-29&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116649221A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |