Method for extracting and determining selenoprotein from selenium-rich lotus leaf lyophyllum commune mycelium

The invention relates to the technical field of biological medicine, in particular to a method for extracting and measuring selenoprotein from selenium-rich lyophyllum decastes mycelium, which comprises the following steps: putting lyophyllum decastes mycelium obtained by fermenting and culturing lyophyllum decastes through a 20L biological culture tank into a vacuum drying oven for drying, grinding into powder, sieving with a 100-mesh sieve to prepare standard powder, weighing 0.5 g of standard powder, and filling the standard powder into a container; adding a NaOH solution with the concentration of 0.05 mol/L, carrying out ultrasonic-assisted extraction, centrifuging at the rotating speed of 3000r/min for 15 minutes, then adjusting the supernate to an isoelectric point, centrifuging again to obtain protein precipitate, and finally drying the protein precipitate in a freeze dryer to obtain a protein sample. According to the method, selenium-rich lyophyllum decastes mycelium is taken as a raw material, ultras