Rapid multicolor imaging method based on fluorescent probe dual-channel brightness coding

The invention discloses a rapid multicolor imaging method based on fluorescent probe dual-channel brightness coding, the method is based on a dual-channel multicolor fluorescent microscopic imaging system, and the method comprises the following steps: S1, determining the characteristics of the dual-channel multicolor fluorescent microscopic imaging system and the spectral characteristics of each optical element; s2, system devices are analyzed, the system is divided into an excitation light path and an emission light path, and the devices in the light paths are coded; s3, coding according to the actual brightness of different fluorescent probes in the system under each excitation wavelength; s4, screening a fluorescent probe supported by the system; s5, judging whether the fluorescent probe meets two-color simultaneous imaging and time-sharing imaging or not; and S6, according to the brightness codes, matching a fluorescent probe combination to realize multicolor imaging. Whether the brightness of the fluores