Taqman-MGB probe and primer for detecting lethal amanita of amanita eaves group and application of Taqman-MGB probe and primer

The invention discloses a Taqman-MGB probe and primer for detecting lethal amanita of an amanita eaves group and application of the Taqman-MGB probe and primer, and belongs to the field of molecular biology. The sequence of the Taqman-MGB probe is as shown in SEQ ID NO. 4, and the sequence of the primer is as shown in SEQ ID NO. 2-3. The Taqman-MGB probe and the primer can be used for identifying the lethal amanita of the amanita amanita group, and tests prove that the Taqman-MGB probe and the primer still have good detection capability on samples which are boiled in water for 60 minutes at least and are subjected to artificial gastric juice simulated digestion for 120 minutes at least, and the lowest detection limit is 10 pg/mu L. Therefore, the method provided by the invention can accurately detect the target species of the sample under complex conditions, provides detection evidence for poisoning events caused by the lethal species in the amanita amanita group, and assists medical units in formulating targ