Linoleic acid synthase gene, cloning method and application of linoleic acid synthase gene

The invention belongs to the technical field of molecular biology and metabolic engineering, and discloses a linoleic acid synthase gene, a cloning method and application of the linoleic acid synthase gene, the nucleotide sequence of the linoleic acid synthase gene FAD2 is SEQ ID NO.1, and the amino...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	GAO HAONAN, AN TIANYUE, LI DEFANG, ZHENG QIUSHENG, WANG GUOLI, MA MENGYU, LI MINGKAI, HONG PAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention belongs to the technical field of molecular biology and metabolic engineering, and discloses a linoleic acid synthase gene, a cloning method and application of the linoleic acid synthase gene, the nucleotide sequence of the linoleic acid synthase gene FAD2 is SEQ ID NO.1, and the amino acid residue sequence of protein encoded by the linoleic acid synthase gene FAD2 is SEQ ID NO.2. The construction method of the saccharomyces cerevisiae engineering strain for biosynthesis of linoleic acid comprises the following steps: taking saccharomyces cerevisiae as an original strain, knocking out FAA4 and FAA1 genes, and overexpressing TGL1, TGL3, TGL5 and FAD2 genes; the TGL1 gene is integrated to the FAA4 gene locus; the TGL3 gene and the TGL5 gene are integrated to the FAA1 gene locus; the FAD2 gene is overlapped with a constitutive promoter TEF1p and a terminator CYC1t, and is integrated to an NDT80 site. The metabolic engineering method for improving the yield of the yeast linoleic acid based on the ov