Cloning and application of potentilla serrata anti-cadmium gene PsMYB62

The invention relates to cloning and application of a potentilla serrata PsMYB62 gene. The length of the nucleotide sequence of the gene is 942bp, the sequence is SEQ ID NO: 1, and the amino acid sequence coded by the gene is SEQ ID NO: 2. According to the invention, a plant expression vector PBI121...

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Bibliographische Detailangaben
Hauptverfasser:	FENG ZHENGHONG, GAO BING, WU JIANHUI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to cloning and application of a potentilla serrata PsMYB62 gene. The length of the nucleotide sequence of the gene is 942bp, the sequence is SEQ ID NO: 1, and the amino acid sequence coded by the gene is SEQ ID NO: 2. According to the invention, a plant expression vector PBI121-PsMYB62-GFP is constructed, and is transferred into a model plant arabidopsis thaliana to obtain an overexpression strain. The function of the PsMYB62 gene is verified under Cd stress, the gene can effectively relieve damage of excessive Cd to plants, and gene resources are provided for cultivation of cadmium-tolerant plants. 本发明涉及一种绢毛委陵菜PsMYB62基因克隆及应用。该基因核苷酸序列长度为942bp，序列为SEQ ID NO:1，该基因编码的氨基酸序列为SEQ ID NO:2。本发明构建了植物表达载体PBI121-PsMYB62-GFP，并转入模式植物拟南芥中，获得过表达株系。在Cd胁迫下验证了PsMYB62基因功能，该基因能够有效缓解过量Cd对植物的损害，为培育耐镉植物提供了基因资源。