Recombinant pichia pastoris genetically engineered bacterium for over-expressing CLB2 as well as construction method and application of recombinant pichia pastoris genetically engineered bacterium

The invention discloses a recombinant pichia pastoris genetically engineered bacterium for over-expressing CLB2 as well as a construction method and application of the recombinant pichia pastoris genetically engineered bacterium. The recombinant pichia pastoris genetically engineered bacterium is characterized in that a cell cycle related gene CLB2 is over-expressed in starting pichia pastoris Komagatapha phaffii GS115-xyn, and the recombinant pichia pastoris genetically engineered bacterium is a recombinant pichia pastoris genetically engineered bacterium. Compared with starting bacteria, the recombinant pichia pastoris genetically engineered bacteria have the advantages that in the fermentation process, the cell cycle of cells is continuously enriched towards the G2/M period along with the increase of fermentation time, the increase of the cells in the G2/M period is beneficial to the enhancement of the film-forming ability of pichia pastoris, and the modified pichia pastoris genetically engineered bacteria